Anticorps Monoclonal anti-EEF2

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E4D5

N° de cat : 67550-1-PBS

Synonymes

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

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Galerie de données de validation

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  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67550-1-Ig (same clone as 67550-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67550-1-Ig (EEF2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67550-1-PBS cible EEF2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEF2 Protéine recombinante Ag13859
• Nom complet: eukaryotic translation elongation factor 2
• Masse moléculaire calculée: 858 aa, 95 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC126259
• Symbole du gène: EEF2
• Identification du gène (NCBI): 1938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.

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