Anticorps Polyclonal de lapin anti-EEFSEC

EEFSEC Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10628-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10628-1-AP (same clone as 10628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10628-1-AP (same clone as 10628-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10628-1-AP (same clone as 10628-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10628-1-PBS cible EEFSEC dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEFSEC Protéine recombinante Ag0980
• Nom complet: eukaryotic elongation factor, selenocysteine-tRNA-specific
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC007933
• Symbole du gène: EEFSEC
• Identification du gène (NCBI): 60678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.

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