Anticorps Recombinant de lapin anti-EFHD2

EFHD2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240185A8

N° de cat : 83264-6-PBS

Synonymes

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8

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Galerie de données de validation

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  WB analysis using 83264-6-RR (same clone as 83264-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83264-6-RR (EFHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83264-6-RR (same clone as 83264-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-6-RR, Clone:240185A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83264-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83264-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83264-6-RR against Human EFHD2 were performed. The affinity constant is 4.63 nM.

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Informations sur le produit

83264-6-PBS cible EFHD2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EFHD2 Protéine recombinante Ag35084
• Nom complet: EF-hand domain family, member D2
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC023611
• Symbole du gène: EFHD2
• Identification du gène (NCBI): 79180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EFHD2 also named Swiprosin-1, is a Ca2+ binding actin protein. The expression of EFHD2 is upregulated during the activation of immune cells, epithelial and endothelial cells. The expression of EFHD2 is regulated by diverse signaling pathways that are contingent upon the specific type of cells.

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