Anticorps Recombinant de lapin anti-EGF

EGF Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242000B1

N° de cat : 84601-1-PBS

Synonymes

242000B1, Epidermal growth factor, Pro-epidermal growth factor

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Galerie de données de validation

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  WB analysis of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84601-1-RR (EGF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84601-1-RR (EGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84601-1-RR (EGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using EGF antibody (84601-1-RR, Clone: 242000B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01451-1

  Cytometric bead array standard curve of MP01451-1, MOUSE EGF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84601-1-PBS. Detection antibody: 84601-2-PBS. Standard: Eg1965. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84601-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84601-1-PBS against Mouse EGF was performed. The affinity constant is 0.671 nM.

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Informations sur le produit

84601-1-PBS cible EGF dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EGF Protéine recombinante Eg1965
• Nom complet: epidermal growth factor
• Masse moléculaire calculée: 133 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_010113
• Symbole du gène: Egf
• Identification du gène (NCBI): 13645
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal Growth Factor (EGF) is a type of mitogenic factor that stimulates the proliferation of various cells, including epithelial cells and fibroblasts. It is a small peptide composed of 53 amino acids with a molecular weight of approximately 6,000 Daltons. EGF contains three disulfide bonds, which contribute to its stability under acidic and high-temperature conditions. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF. EGF plays a crucial role in regulating cell growth, proliferation, and differentiation. When EGF binds to its receptor (EGFR), it activates intracellular signaling pathways that lead to DNA synthesis and cell proliferation. EGF is naturally present in various tissues and body fluids, including saliva, urine, and milk. It is primarily synthesized in the submandibular glands and duodenum.

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