Anticorps Monoclonal anti-EGFR

• Phare
• Validé par KD/KO

EGFR Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

51

CloneNo.

2A2H10

Avis (4)

N° de cat : 66455-1-Ig

Synonymes

EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

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Galerie de données de validation

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  WB analysis using 66455-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66455-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66455-1-Ig

  WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells.

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  WB analysis of EC109 using 66455-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66455-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66455-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66455-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66455-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66455-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66455-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 66455-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 66455-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human EGFR (66455-1-Ig, Clone:2A2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules EC109, cellules HeLa, cellules HepG2, cellules LNCaP, cellules MDA-MB-231, cellules MDA-MB-468, cellules PC-3, cellules PC-3, cellules SKOV-3
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la peau humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 45 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Informations sur le produit

66455-1-Ig cible EGFR dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EGFR Protéine recombinante Ag24947
• Nom complet: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Masse moléculaire calculée: 1210 aa, 134 kDa
• Poids moléculaire observé: 145-165 kDa
• Numéro d’acquisition GenBank: BC094761
• Symbole du gène: EGFR
• Identification du gène (NCBI): 1956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

Publications

Species	Application	Title
human	WB	Mol Cell; N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.; Authors - Zihao Dai ; KD Validated
human	WB,IHC	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu
human	WB,IHC	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
human	IHC	Cell Death Dis; Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR.; Authors - Xiao-Wei Zhang
human	WB	Phytomedicine; Maintenance of airway epithelial barrier integrity via the inhibition of AHR/EGFR activation ameliorates chronic obstructive pulmonary disease using effective-component combination; Authors - Yanxin Wei
human	WB,IF	J Transl Med; Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1; Authors - Ruijiao Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

k. (Verified Customer) (10-26-2023)

This antibody worked well for human cells and mouse liver cell proteins at 1:500 or 1:1000 concentrations at 4 °C over a night of incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

FH

Christos (Verified Customer) (02-13-2023)

-35ug protein extract were loaded per well -Transfer was performed for 2hr at 400mA at 4oC, on a Nitrocellulose Blotting Membrane -Membrane blocking was performed in 5% non-fat milk in PBS-Tween20 at room temperature, under mild shacking -Antibodies dilutions were performed in 5% non-fat milk in PBS-Tween20. -Incubations with the primary antibodies were performed as followed: 1)EGFR: 1:1000 for 1.5hr at 4oC 2)tubulin (sc32293, Santa Cruz): 1:5000 for 1.5hr at 4oC -Incubations with the secondary antibodies were performed with Rb pAb to Ms IgG (HRP) (ab 6728, Abcam) at a 1:20000 dilution, for 1hr at 4oC.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human breast cancer cells/ SKBR3, MDA-MB-231

EGFR Antibody Western Blot validation (1/1000 dilution) in Human breast cancer cells/ SKBR3, MDA-MB-231 (Cat no:66455-1-Ig)

FH

Guorong (Verified Customer) (03-31-2022)

A band of approximately 160 kDa was detected

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ARPE-19

EGFR Antibody Western Blot validation (1:1000 dilution) in ARPE-19 (Cat no:66455-1-Ig)

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other