Anticorps Monoclonal anti-EGFR

• Phare
• Validé par KD/KO

EGFR Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A2H10

N° de cat : 66455-1-PBS

Synonymes

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2A2H10

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Galerie de données de validation

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  WB analysis using 66455-1-Ig (same clone as 66455-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66455-1-Ig (same clone as 66455-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)

  WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  WB analysis of EC109 using 66455-1-Ig (same clone as 66455-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66455-1-Ig (same clone as 66455-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66455-1-Ig (same clone as 66455-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66455-1-Ig (same clone as 66455-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 66455-1-Ig (same clone as 66455-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66455-1-Ig (same clone as 66455-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66455-1-Ig (same clone as 66455-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66455-1-PBS cible EGFR dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EGFR Protéine recombinante Ag24947
• Nom complet: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Masse moléculaire calculée: 1210 aa, 134 kDa
• Poids moléculaire observé: 145-165 kDa
• Numéro d’acquisition GenBank: BC094761
• Symbole du gène: EGFR
• Identification du gène (NCBI): 1956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

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