Anticorps Polyclonal de lapin anti-PHD2/EGLN1

• Phare
• Validé par KD/KO

PHD2/EGLN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 19886-1-AP

Synonymes

EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3

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Galerie de données de validation

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  WB analysis using 19886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19886-1-AP

  IP result of anti-PHD2/EGLN1 (IP:19886-1-AP, 4ug; Detection:19886-1-AP 1:5000) with HEK-293 cells lysate 1280 ug.

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  IHC staining of human liver cancer using 19886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 19886-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PHD2/EGLN1 antibody (19886-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HEK-293T, cellules HepG2, tissu cardiaque de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

19886-1-AP cible PHD2/EGLN1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHD2/EGLN1 Protéine recombinante Ag13706
• Nom complet: egl nine homolog 1 (C. elegans)
• Masse moléculaire calculée: 426 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC005369
• Symbole du gène: PHD2/EGLN1
• Identification du gène (NCBI): 54583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao ; KO Validated
mouse	WB	Adv Sci (Weinh); Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway; Authors - Ling-Na Zhao
	WB,IHC	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
	WB	Cell Mol Life Sci; Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway.; Authors - Chang-Chun Song
human	WB	Int J Mol Sci; Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α.; Authors - Kenta Kaihara
mouse	WB	Biomed Pharmacother; A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue.; Authors - Shilei Zhang

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