Anticorps Polyclonal de lapin anti-PHD1

PHD1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12984-1-AP

Synonymes

Egl nine homolog 2, EGLN2, EIT6, Estrogen induced tag 6, HIF PH1, HIF prolyl hydroxylase 1, HIFPH1, HPH 1, HPH 3, PHD1

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Galerie de données de validation

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  WB analysis of mouse testis using 12984-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12984-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12984-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12984-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12984-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 12984-1-AP

  Immunofluorescent analysis of PC-3 cells using 12984-1-AP (PHD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HEK-293, cellules HeLa, cellules PC-3, tissu cardiaque de souris, tissu splénique humain
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

12984-1-AP cible PHD1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHD1 Protéine recombinante Ag3616
• Nom complet: egl nine homolog 2 (C. elegans)
• Masse moléculaire calculée: 407 aa, 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC036051
• Symbole du gène: EGLN2
• Identification du gène (NCBI): 112398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	Respir Res; Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.; Authors - Changhui Yu
rat	IF	J Cell Mol Med; LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin-mediated degradation.; Authors - Suping Li
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang
human	IF	Leuk Lymphoma; Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.; Authors - Rintaro Ohe
human	WB	Cell Death Dis; Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis.; Authors - Haixu Xu