Anticorps Polyclonal de lapin anti-PHD1

PHD1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 12984-1-AP

Synonymes

EGLN2, EC:1.14.11.-, EC:1.14.11.29, EIT6, EIT-6

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Galerie de données de validation

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  WB analysis of mouse testis using 12984-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12984-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12984-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12984-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12984-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HEK-293, cellules HeLa, cellules PC-3, tissu cardiaque de souris, tissu splénique humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12984-1-AP cible PHD1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHD1 Protéine recombinante Ag3616
• Nom complet: egl nine homolog 2 (C. elegans)
• Masse moléculaire calculée: 407 aa, 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC036051
• Symbole du gène: PHD1
• Identification du gène (NCBI): 112398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	Respir Res; Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.; Authors - Changhui Yu
mouse	WB,IHC	Am J Pathol; Genetic Loss of HIF-Prolyl-Hydroxylase 1, but Not Pharmacological Inhibition, Mitigates Hepatic Fibrosis; Authors - Christopher Tuffs
rat	IF	J Cell Mol Med; LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin-mediated degradation.; Authors - Suping Li
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang
human	IF	Leuk Lymphoma; Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.; Authors - Rintaro Ohe

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