Anticorps Polyclonal de lapin anti-PHD3

• Phare
• Validé par KD/KO

PHD3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 18325-1-AP

Synonymes

Egl nine homolog 3, EGLN3, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3

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Galerie de données de validation

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  WB analysis using 18325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18325-1-AP (PHD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 18325-1-AP

  WB result of PHD3 antibody (18325-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHD3 transfected HEK-293T cells.

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  IP experiment of A375 using 18325-1-AP

  IP Result of anti-PHD3 (IP:18325-1-AP, 4ug; Detection:18325-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HEK-293, cellules HEK-293T, cellules HT-1080, tissu placentaire humain
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

18325-1-AP cible PHD3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHD3 Protéine recombinante Ag13197
• Nom complet: egl nine homolog 3 (C. elegans)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-32 kDa
• Numéro d’acquisition GenBank: BC010992
• Symbole du gène: EGLN3
• Identification du gène (NCBI): 112399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	IHC	Cancer Res; Prolyl Hydroxylase 3 Attenuates MCL-1-Mediated ATP Production to Suppress the Metastatic Potential of Colorectal Cancer Cells.; Authors - Praveenkumar Radhakrishnan
human	WB	Mol Ther; WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.; Authors - Tianli Chen ; KD Validated
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang
human	WB	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng
human	IHC	Med Oncol; Overexpression of the HIF hydroxylase PHD3 is a favorable prognosticator for gastric cancer.; Authors - Su Changlei C