Anticorps Recombinant de lapin anti-EGLN3

EGLN3 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240165A6

N° de cat : 83321-1-PBS

Synonymes

PHD3, 240165A6, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3

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Galerie de données de validation

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  IF Staining of HepG2 using 83321-1-RR (same clone as 83321-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3 antibody (83321-1-RR, Clone: 240165A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83321-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83321-1-RR (same clone as 83321-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83321-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83321-1-RR (same clone as 83321-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83321-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00347-1

  Cytometric bead array standard curve of MP00347-1, EGLN3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83321-1-PBS. Detection antibody: 83321-4-PBS. Standard: Ag27464. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83321-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83321-1-PBS against Human EGLN3 was performed. The affinity constant is 4.82 nM.

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  Affinity and Kinetic Characterization of 83321-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83321-1-RR against Human EGLN3 were performed. The affinity constant is 1.82 nM.

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Informations sur le produit

83321-1-PBS cible EGLN3 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EGLN3 Protéine recombinante Ag27464
• Nom complet: egl nine homolog 3 (C. elegans)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC010992
• Symbole du gène: EGLN3
• Identification du gène (NCBI): 112399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

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