Anticorps Recombinant de lapin anti-EGLN3

EGLN3 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1O19

N° de cat : 82377-1-PBS

Synonymes

EGLN3/PHD3, PHD3, 1O19, EC:1.14.11.-, EC:1.14.11.29

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Galerie de données de validation

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  WB analysis using 82377-1-RR (same clone as 82377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82377-1-RR (EGLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82377-1-RR (same clone as 82377-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82377-1-RR (same clone as 82377-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82377-1-RR (same clone as 82377-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug EGLN3/PHD3 Recombinant antibody (82377-1-RR, Clone:1O19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82377-1-PBS cible EGLN3 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EGLN3 Protéine recombinante Ag13197
• Nom complet: egl nine homolog 3 (C. elegans)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC010992
• Symbole du gène: EGLN3
• Identification du gène (NCBI): 112399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

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