Anticorps Recombinant de lapin anti-EHD2

EHD2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243038C6

N° de cat : 85604-4-RR

Synonymes

EH domain containing 2, EH domain containing protein 2, EH domain-containing protein 2, PAST homolog 2, PAST2

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Galerie de données de validation

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  WB analysis using 85604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85604-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85604-4-RR against Human EHD2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules MDA-MB-231, tissu cardiaque de rat, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85604-4-RR cible EHD2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EHD2 Protéine recombinante Ag2002
• Nom complet: EH-domain containing 2
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC014445
• Symbole du gène: EHD2
• Identification du gène (NCBI): 30846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.

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