Anticorps Polyclonal de lapin anti-EHHADH

EHHADH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 26570-1-AP

Synonymes

3-hydroxyacyl-CoA dehydrogenase, ECHD, Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase, L PBE, LBFP

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Galerie de données de validation

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  WB analysis using 26570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 26570-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of HepG2 using 26570-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26570-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26570-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26570-1-AP (EHHADH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 26570-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26570-1-AP (EHHADH Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 26570-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26570-1-AP (EHHADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HepG2, cellules SMMC-7721, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

26570-1-AP cible EHHADH dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EHHADH Protéine recombinante Ag24237
• Nom complet: enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
• Masse moléculaire calculée: 723 aa, 79 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC038948
• Symbole du gène: EHHADH
• Identification du gène (NCBI): 1962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
mouse	WB	Cell Rep; Feedback repression of PPARα signaling by Let-7 microRNA.; Authors - Tomoki Yagai
mouse	WB	Int J Mol Sci; Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis; Authors - Yingli Zhu
mouse	WB	Int J Biochem Cell Biol; Tamoxifen upregulates the peroxisomal β-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice; Authors - Ziling Zhang
mouse	WB	Geroscience; Coordinated transcriptional upregulation of oxidative metabolism proteins in long-lived endocrine mutant mice; Authors - Ahmed M Elmansi

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