Anticorps Polyclonal de lapin anti-EIF1AX

EIF1AX Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 14654-1-AP

Synonymes

eIF-1A X isoform, eIF1A X isoform, EIF1A, eIF 4C, eIF 1A X isoform

---

Galerie de données de validation

• 

  WB analysis using 14654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HepG2 using 14654-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14654-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 14654-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14654-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1AX antibody (14654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A375, cellules HeLa, cellules PC-3
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14654-1-AP cible EIF1AX dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF1AX Protéine recombinante Ag6253
• Nom complet: eukaryotic translation initiation factor 1A, X-linked
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16-23 kDa
• Numéro d’acquisition GenBank: BC067851
• Symbole du gène: EIF1AX
• Identification du gène (NCBI): 1964
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.

Publications

Species	Application	Title
		Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Proteomics; Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.; Authors - Hao-Yu Li

{{ptg:RelatedPrimaryAntibodies}}