Anticorps Polyclonal de lapin anti-EIF1AX

EIF1AX Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14654-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14654-1-AP (same clone as 14654-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 14654-1-AP (same clone as 14654-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1AX antibody (14654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14654-1-PBS cible EIF1AX dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF1AX Protéine recombinante Ag6253
• Nom complet: eukaryotic translation initiation factor 1A, X-linked
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16-23 kDa
• Numéro d’acquisition GenBank: BC067851
• Symbole du gène: EIF1AX
• Identification du gène (NCBI): 1964
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.

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