Anticorps Polyclonal de lapin anti-EIF1AY

• Phare
• Validé par KD/KO

EIF1AY Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 11193-1-AP

Synonymes

Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-4C, eIF-1A Y isoform, eIF1A Y isoform, eIF 4C

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 11193-1-AP

  WB result of EIF1AY antibody (11193-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF1AY transfected MCF-7 cells.

• 

  WB analysis of MCF-7 using 11193-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11193-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 11193-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11193-1-AP (EIF1AY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules MCF-7
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11193-1-AP cible EIF1AY dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF1AY Protéine recombinante Ag1680
• Nom complet: eukaryotic translation initiation factor 1A, Y-linked
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC005248
• Symbole du gène: EIF1AY
• Identification du gène (NCBI): 9086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF1AY is 1 of 5 NRY genes that are expressed in many tissues and have homologs on the X chromosome, which is an essential translation initiation factor. EIF1AY seems to be required for maximal rate of protein biosynthesis, and enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. This is a rabbit polyclonal antibody raised against the full length of human EIF1AY, and can recognize both EIF1AY and EIF1AX.

{{ptg:RelatedPrimaryAntibodies}}