Anticorps Monoclonal anti-EIF2A/CDA02

• Phare
• Validé par KD/KO

EIF2A/CDA02 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G7H4

N° de cat : 66482-1-PBS

Synonymes

EIF2A, 1G7H4, 65 kDa eukaryotic translation initiation factor 2A, CDA02, EIF

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Galerie de données de validation

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  WB analysis of HEK-293 using 66482-1-Ig (same clone as 66482-1-PBS)

  WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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  WB analysis using 66482-1-Ig (same clone as 66482-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66482-1-Ig (same clone as 66482-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66482-1-Ig (same clone as 66482-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF2A/CDA02 antibody (66482-1-Ig, Clone: 1G7H4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66482-1-PBS cible EIF2A/CDA02 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF2A/CDA02 Protéine recombinante Ag22041
• Nom complet: eukaryotic translation initiation factor 2A, 65kDa
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011885
• Symbole du gène: EIF2A
• Identification du gène (NCBI): 83939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.

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