Anticorps Polyclonal de lapin anti-EIF2AK1

• Phare
• Validé par KD/KO

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20499-1-PBS

Synonymes

EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor, Heme regulated inhibitor

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 20499-1-AP (same clone as 20499-1-PBS)

  WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20499-1-AP (same clone as 20499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20499-1-AP (same clone as 20499-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20499-1-AP (same clone as 20499-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

20499-1-PBS cible EIF2AK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2AK1 Protéine recombinante Ag14236
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 1
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC006524
• Symbole du gène: EIF2AK1
• Identification du gène (NCBI): 27102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

{{ptg:RelatedPrimaryAntibodies}}