Anticorps Monoclonal anti-EIF2AK1

EIF2AK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H1F3

N° de cat : 67674-1-PBS

Synonymes

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

---

Galerie de données de validation

• 

  WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67674-1-Ig (same clone as 67674-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67674-1-PBS cible EIF2AK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF2AK1 Protéine recombinante Ag16970
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 1
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC006524
• Symbole du gène: EIF2AK1
• Identification du gène (NCBI): 27102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

{{ptg:RelatedPrimaryAntibodies}}