Anticorps Recombinant de lapin anti-EIF2AK1

• Phare
• Validé par KD/KO

EIF2AK1 Recombinant Antibody for Indirect ELISA, WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Indirect ELISA, WB

Conjugaison

Non conjugué

CloneNo.

3J9

N° de cat : 81768-1-PBS

Synonymes

Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 81768-1-RR (EIF2AK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81768-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

  WB result of EIF2AK1 antibody (81768-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 81768-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81768-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81768-1-RR against Human EIF2AK1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81768-1-PBS cible EIF2AK1 dans les applications de Indirect ELISA, WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EIF2AK1 Protéine recombinante Ag14236
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 1
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC006524
• Symbole du gène: EIF2AK1
• Identification du gène (NCBI): 27102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

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