Anticorps Polyclonal de lapin anti-PERK/EIF2AK3

PERK/EIF2AK3 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

81

N° de cat : 24390-1-AP

Synonymes

EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS

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Galerie de données de validation

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  WB analysis using 24390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 24390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 24390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human liver cancer using 24390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens)..

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  FC experiment of HeLa using 24390-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (24390-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 75 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

24390-1-AP cible PERK/EIF2AK3 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PERK/EIF2AK3 Protéine recombinante Ag18254
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 3
• Masse moléculaire calculée: 1116 aa, 125 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC126354
• Symbole du gène: EIF2AK3
• Identification du gène (NCBI): 9451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.; Authors - Feixiang Yuan
		J Adv Res; Postantibiotic leukocyte enhancement-mediated reduction of intracellular bacteria by macrophages; Authors - Yifan Wu
human	WB	Autophagy; Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.; Authors - Tengfei Huang
pig	WB	Autophagy; STING1 is essential for an RNA-virus triggered autophagy.; Authors - Rui Zhang ; KD Validated
pig	WB	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming
mouse	WB	Br J Pharmacol; Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation; Authors - Hang Su