Anticorps Polyclonal de lapin anti-EIF2B1

• Phare
• Validé par KD/KO

EIF2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18010-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of MCF-7 using 18010-1-AP (same clone as 18010-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 18010-1-AP (same clone as 18010-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18010-1-AP (same clone as 18010-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 18010-1-AP (same clone as 18010-1-PBS)

  IP result of anti-EIF2B1 (IP:18010-1-AP, 4ug; Detection:18010-1-AP 1:700) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18010-1-AP (same clone as 18010-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2B1 antibody (18010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18010-1-PBS cible EIF2B1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2B1 Protéine recombinante Ag12509
• Nom complet: eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
• Masse moléculaire calculée: 305 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC103763
• Symbole du gène: EIF2B1
• Identification du gène (NCBI): 1967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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