Anticorps Polyclonal de lapin anti-EIF2B5

EIF2B5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29935-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29935-1-AP (same clone as 29935-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29935-1-AP (EIF2B5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29935-1-AP (same clone as 29935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29935-1-AP (same clone as 29935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 29935-1-AP (same clone as 29935-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 29935-1-AP (same clone as 29935-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29935-1-PBS cible EIF2B5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2B5 Protéine recombinante Ag32175
• Nom complet: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 65-80 kDa
• Numéro d’acquisition GenBank: NM_003907.2
• Symbole du gène: EIF2B5
• Identification du gène (NCBI): 8893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF2B5 encodes translation initiation factor eIF-2B subunit epsilon protein (PMID 8688466). EIF2B5 mutations compromise the induction of GFAP-expressing astrocytes in VWM (vanishing white matter) leukodystrophy (PMID:15723074, PMID:15776425). The upregulation of EIF2B5 is associated with cancer such as human pulmonary adenocarcinoma cell and breast cancer (PMID:24366584, PMID:24979338). EIF2B5 also has 65kDa truncated protein isoform (PMID:28961236).

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