Anticorps Monoclonal anti-EIF2S1

• Phare
• Validé par KD/KO

EIF2S1 Monoclonal Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C8E2

N° de cat : 68479-1-PBS

Synonymes

EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha

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Galerie de données de validation

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  WB analysis using 68479-1-Ig (same clone as 68479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68479-1-Ig (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68479-1-Ig (same clone as 68479-1-PBS)

  WB result of EIF2S1 antibody (68479-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2S1 transfected HepG2 cells. This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 68479-1-Ig (same clone as 68479-1-PBS)

  IP result of anti-EIF2S1 (IP:68479-1-Ig, 4ug; Detection:68479-1-Ig 1:4000) with HepG2 cells lysate 1480 ug. This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68479-1-Ig (same clone as 68479-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (68479-1-Ig, Clone: 3C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50181-1

  Cytometric bead array in cell lysate using MP50181-1, Phospho-EIF2S1 (Ser51) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68479-1-PBS. Detection antibody: 68023-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Informations sur le produit

68479-1-PBS cible EIF2S1 dans les applications de WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF2S1 Protéine recombinante Ag28309
• Nom complet: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002513
• Symbole du gène: EIF2S1
• Identification du gène (NCBI): 1965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

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