Anticorps Monoclonal anti-EIF2S2

EIF2S2 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H8E1

N° de cat : 68463-1-PBS

Synonymes

2H8E1, eIF 2 beta, EIF2, EIF2B, EIF2beta

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Galerie de données de validation

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  WB analysis using 68463-1-Ig (same clone as 68463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68463-1-Ig (EIF2S2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68463-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68463-1-PBS cible EIF2S2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF2S2 Protéine recombinante Ag18349
• Nom complet: eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC000461
• Symbole du gène: EIF2S2
• Identification du gène (NCBI): 8894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.

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