Anticorps Polyclonal de lapin anti-EIF2S3

EIF2S3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11162-1-AP

Synonymes

eIF 2 gamma X, eIF 2gA, eIF 2gX, EIF2, EIF2G, EIF2gamma, EIF2S3

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Galerie de données de validation

• 

  WB analysis of HeLa using 11162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11162-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11162-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11162-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11162-1-AP

  IP Result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug.

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11162-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, tissu de thymus de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11162-1-AP cible EIF2S3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2S3 Protéine recombinante Ag1650
• Nom complet: eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC019906
• Symbole du gène: EIF2S3
• Identification du gène (NCBI): 1968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]

Publications

Species	Application	Title
human	WB	Nat Neurosci; Deletion of TOP3β, a component of FMRP-containing mRNPs, contributes to neurodevelopmental disorders.; Authors - Stoll Georg G
human	WB	Mol Biol Cell; PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation.; Authors - Nasim Haghandish