Anticorps Polyclonal de lapin anti-EIF2S3

EIF2S3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11162-1-PBS

Synonymes

EIF2G, EIF2, eIF 2gX, eIF 2gA, eIF 2 gamma X

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Galerie de données de validation

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  WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  IP result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2S3 antibody (11162-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11162-1-PBS cible EIF2S3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2S3 Protéine recombinante Ag1650
• Nom complet: eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC019906
• Symbole du gène: EIF2S3
• Identification du gène (NCBI): 1968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]

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