Anticorps Polyclonal de lapin anti-EIF3A

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 26178-1-AP

Synonymes

eIF3 p185, eIF3 p180, eIF3 p170, eIF3 p167, EIF3

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Galerie de données de validation

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  WB analysis of HEK-293 using 26178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 26178-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF3A antibody (26178-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

26178-1-AP cible EIF3A dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF3A Protéine recombinante Ag22515
• Nom complet: eukaryotic translation initiation factor 3, subunit A
• Masse moléculaire calculée: 1382 aa, 166 kDa
• Poids moléculaire observé: 166 kDa
• Numéro d’acquisition GenBank: BC114429
• Symbole du gène: EIF3A
• Identification du gène (NCBI): 8661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
human	WB,IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo
human	WB	Onco Targets Ther; WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.; Authors - Jingjing Dai
mouse	WB	J Clin Lab Anal; Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.; Authors - Zhenwei Li
human	RIP	Proc Natl Acad Sci U S A; Epitranscriptic regulation of HRAS by N6-methyladenosine drives tumor progression; Authors - Yongbo Pan
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi

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