Anticorps Polyclonal de lapin anti-EIF3B

EIF3B Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 10319-1-AP

Synonymes

eIF 3 eta, EIF3 ETA, EIF3 P110, EIF3 P116, EIF3B, EIF3S9, hPrt1, PRT1, Prt1 homolog

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Galerie de données de validation

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  WB analysis of A375 using 10319-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10319-1-AP (EIF3B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 10319-1-AP

  IP result of anti-EIF3B (IP:10319-1-AP, 4ug; Detection:10319-1-AP 1:300) with A375 cells lysate 3600ug.

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Applications testées

• Résultats positifs en WB: cellules A375
• Résultats positifs en IP: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

10319-1-AP cible EIF3B dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF3B Protéine recombinante Ag0386
• Nom complet: eukaryotic translation initiation factor 3, subunit B
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC001173
• Symbole du gène: EIF3B
• Identification du gène (NCBI): 8662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF3B, also named as Eukaryotic translation initiation factor 3 subunit B, is a 814 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and 8 WD repeats and belongs to the eIF-3 subunit B family. EIF3B as a RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. The calculated molecular weight of EIF3B is 93 kDa, but the phosphorylated EIF3B protein is about 115 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB	Elife; Translation initiation by the hepatitis C virus IRES requires eIF1A and ribosomal complex remodeling.; Authors - Zane A Jaafar
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio
mouse	WB	bioRxiv; Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.; Authors - Li Wang
human	WB	Nat Commun; METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions; Authors - Yuanpei Li
		Cell Chem Biol; Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation; Authors - Adil Muneer

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