Anticorps Monoclonal anti-EIF3D

EIF3D Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2C5A3

N° de cat : 66024-1-Ig

Synonymes

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

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Galerie de données de validation

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  WB analysis of HepG2 using 66024-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66024-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66024-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66024-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66024-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66024-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66024-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 66024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 66024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66024-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66024-1-Ig cible EIF3D dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF3D Protéine recombinante Ag18092
• Nom complet: eukaryotic translation initiation factor 3, subunit D
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC000328
• Symbole du gène: EIF3D
• Identification du gène (NCBI): 8664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.