Anticorps Monoclonal anti-EIF3D

• Phare
• Validé par KD/KO

EIF3D Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C5A3

N° de cat : 66024-1-PBS

Synonymes

eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta

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Galerie de données de validation

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  WB analysis of HepG2 using 66024-1-Ig (same clone as 66024-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66024-1-Ig (same clone as 66024-1-PBS)

  WB result of EIF3D antibody (66024-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF3D transfected HEK-293 cells. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 66024-1-Ig (same clone as 66024-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66024-1-Ig (same clone as 66024-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66024-1-Ig (same clone as 66024-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66024-1-PBS cible EIF3D dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF3D Protéine recombinante Ag18092
• Nom complet: eukaryotic translation initiation factor 3, subunit D
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC000328
• Symbole du gène: EIF3D
• Identification du gène (NCBI): 8664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

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