Anticorps Monoclonal anti-EIF3E

EIF3E Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C6C8

N° de cat : 67095-1-PBS

Synonymes

1C6C8, eIF-3 p48, EIF3S6, INT6, Viral integration site protein INT-6 homolog

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Galerie de données de validation

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  WB analysis using 67095-1-Ig (same clone as 67095-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67095-1-Ig (same clone as 67095-1-PBS)

  IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67095-1-Ig (same clone as 67095-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67095-1-PBS cible EIF3E dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF3E Protéine recombinante Ag28523
• Nom complet: eukaryotic translation initiation factor 3, subunit E
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-52 kDa
• Numéro d’acquisition GenBank: BC000734
• Symbole du gène: EIF3E
• Identification du gène (NCBI): 3646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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