Anticorps Recombinant de lapin anti-EIF3F

EIF3F Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240894A6

N° de cat : 84131-5-PBS

Synonymes

eIF3 p47, 240894A6

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Galerie de données de validation

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  WB analysis using 84131-5-RR (same clone as 84131-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84131-5-RR (EIF3F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84131-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84131-5-RR (same clone as 84131-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-5-RR, Clone:240894A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84131-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84131-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84131-5-RR against Human EIF3F were performed. The affinity constant is 0.474 nM.

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Informations sur le produit

84131-5-PBS cible EIF3F dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EIF3F Protéine recombinante Ag34849
• Nom complet: eukaryotic translation initiation factor 3, subunit F
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC000490
• Symbole du gène: EIF3F
• Identification du gène (NCBI): 8665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF3F (eukaryotic translation initiation factor 3 subunit F) is a protein that plays an important role in eukaryotic cells and is an integral part of the translation initiation factor 3(Eif3) complex. EIF3 is a complex of at least 10 distinct subunits and is the largest translation initiation factor in eukaryotes.

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