Anticorps Polyclonal de lapin anti-EIF3J

• Phare
• Validé par KD/KO

EIF3J Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10439-1-AP

Synonymes

eIF 3 alpha, eIF3 alpha, eIF3 p35, EIF3J, EIF3S1

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Galerie de données de validation

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  WB analysis of HeLa using 10439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10439-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10439-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10439-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10439-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10439-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10439-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10439-1-AP

  IP Result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug.

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  IHC staining of human breast cancer using 10439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:10 (under 10x lens).

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  IF Staining of HeLa using 10439-1-AP

  Immunofluorescent analysis of Hela cells, using EIF3J antibody 10439-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules Jurkat, cellules K-562, cellules MCF-7, cellules SKOV-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10439-1-AP cible EIF3J dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF3J Protéine recombinante Ag0678
• Nom complet: eukaryotic translation initiation factor 3, subunit J
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002719
• Symbole du gène: EIF3J
• Identification du gène (NCBI): 8669
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).

Publications

Species	Application	Title
human	WB,IF,IP	J Cell Biol; eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy.; Authors - Ramírez-Valle Francisco F ; KD Validated
human	IF	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas
human	WB	bioRxiv; PARP10 is Critical for Stress Granule Initiation; Authors - Aravinth Kumar Jayabalan