Anticorps Monoclonal anti-EIF3M

EIF3M Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A12B2

N° de cat : 66025-1-PBS

Synonymes

PCID1, 2A12B2, B5, EIF3, GA17

---

Galerie de données de validation

• 

  WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66025-1-Ig (same clone as 66025-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66025-1-Ig (same clone as 66025-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF3M antibody (66025-1-Ig, Clone: 2A12B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using EIF3M antibody 66025-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66025-1-PBS cible EIF3M dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF3M Protéine recombinante Ag17965
• Nom complet: eukaryotic translation initiation factor 3, subunit M
• Masse moléculaire calculée: 374 aa, 43 kDa
• Poids moléculaire observé: 35-43 kDa
• Numéro d’acquisition GenBank: BC019103
• Symbole du gène: EIF3M
• Identification du gène (NCBI): 10480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.

{{ptg:RelatedPrimaryAntibodies}}