Anticorps Monoclonal anti-EIF4A2

EIF4A2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D11E11

N° de cat : 67799-1-PBS

Synonymes

EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010

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Galerie de données de validation

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  WB analysis using 67799-1-Ig (same clone as 67799-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67799-1-PBS cible EIF4A2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF4A2 Protéine recombinante Ag9085
• Nom complet: eukaryotic translation initiation factor 4A, isoform 2
• Masse moléculaire calculée: 408 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC012547
• Symbole du gène: EIF4A2
• Identification du gène (NCBI): 1974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

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