Anticorps Polyclonal de lapin anti-EIF4A3

• Phare
• Validé par KD/KO

EIF4A3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

39

N° de cat : 17504-1-AP

Synonymes

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

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Galerie de données de validation

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  WB analysis of A549 using 17504-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17504-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17504-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17504-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17504-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17504-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17504-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17504-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17504-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17504-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 17504-1-AP

  IP Result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.

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  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 17504-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu cardiaque de souris, tissu de thymus de souris, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 25 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• RIP: See 8 publications below

Informations sur le produit

17504-1-AP cible EIF4A3 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF4A3 Protéine recombinante Ag11701
• Nom complet: eukaryotic translation initiation factor 4A, isoform 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC003662
• Symbole du gène: EIF4A3
• Identification du gène (NCBI): 9775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
		Cell Res; YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.; Authors - Hailing Shi
human	IF,WB	Cell Death Differ; eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.; Authors - Despoina Sakellariou ; KD Validated
human	WB	Mol Cancer; Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.; Authors - Huizhe Wu
human	WB	J Nanobiotechnology; Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling; Authors - Xiao Liu
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis