Anticorps Monoclonal anti-EIF4E3

EIF4E3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E9C4

N° de cat : 68171-1-PBS

Synonymes

eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3

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Galerie de données de validation

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  WB analysis using 68171-1-Ig (same clone as 68171-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68171-1-Ig (EIF4E3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68171-1-PBS cible EIF4E3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF4E3 Protéine recombinante Ag11056
• Nom complet: eukaryotic translation initiation factor 4E family member 3
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24-30 kDa
• Numéro d’acquisition GenBank: BC031289
• Symbole du gène: EIF4E3
• Identification du gène (NCBI): 317649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918).

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