Anticorps Polyclonal de lapin anti-EIF4G3

EIF4G3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11281-1-PBS

Synonymes

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

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Galerie de données de validation

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  WB analysis using 11281-1-AP (same clone as 11281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11281-1-AP (same clone as 11281-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

  IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11281-1-AP (same clone as 11281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11281-1-PBS cible EIF4G3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF4G3 Protéine recombinante Ag1811
• Nom complet: eukaryotic translation initiation factor 4 gamma, 3
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 70 kDa, 250 kDa
• Numéro d’acquisition GenBank: BC030578
• Symbole du gène: EIF4G3
• Identification du gène (NCBI): 8672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.

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