Anticorps Polyclonal de lapin anti-EIF5A

EIF5A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11309-1-AP

Synonymes

eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1, EIF5A, EIF5A1, eIF5AI, Rev binding factor

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Galerie de données de validation

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  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11309-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF5A antibody (11309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11309-1-AP

  Immunofluorescent analysis of HeLa cells using 11309-1-AP (EIF5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, cellules PC-3, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11309-1-AP cible EIF5A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF5A Protéine recombinante Ag1849
• Nom complet: eukaryotic translation initiation factor 5A
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC001832
• Symbole du gène: EIF5A
• Identification du gène (NCBI): 1984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

Publications

Species	Application	Title
human	WB,IHC	J Cancer; EIF5A expression and its role as a potential diagnostic biomarker in hepatocellular carcinoma.; Authors - Linjing Li
mouse	IF	J Ovarian Res; Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes; Authors - Chuanxin Zhang
human	WB	Oncol Lett; SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A; Authors - Boya Deng
human	WB	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated