Anticorps Monoclonal anti-EIF5A

EIF5A Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D1B6

N° de cat : 67214-1-Ig

Synonymes

2D1B6, eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1

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Galerie de données de validation

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  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67214-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 67214-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HaCaT, cellules HEK-293, cellules HEK-293T, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, cellules PC-3, cellules RAW 264.7
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67214-1-Ig cible EIF5A dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF5A Protéine recombinante Ag28588
• Nom complet: eukaryotic translation initiation factor 5A
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC001832
• Symbole du gène: EIF5A
• Identification du gène (NCBI): 1984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

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