Anticorps Polyclonal de lapin anti-EIF6

• Phare
• Validé par KD/KO

EIF6 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10291-1-AP

Synonymes

b(2)gcn, B(2)GCN homolog, B4 integrin interactor, CAB, eIF 6, EIF3A, EIF6, gcn, integrin beta 4 binding protein, ITGB4BP, p27(BBP), p27BBP

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Galerie de données de validation

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  WB analysis of HeLa using 10291-1-AP

  WB result of EIF6 antibody (10291-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF6 transfected HeLa cells.

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  WB analysis using 10291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10291-1-AP (EIF6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10291-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10291-1-AP (EIF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10291-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10291-1-AP (EIF6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10291-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10291-1-AP (EIF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10291-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF6 antibody (10291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules COLO 320, cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

10291-1-AP cible EIF6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF6 Protéine recombinante Ag0324
• Nom complet: eukaryotic translation initiation factor 6
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001119
• Symbole du gène: EIF6
• Identification du gène (NCBI): 3692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), It functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.

Publications

Species	Application	Title
human	WB	Nature; MicroRNA silencing through RISC recruitment of eIF6.; Authors - Chendrimada Thimmaiah P TP ; KD Validated
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
human	WB	Biochem Biophys Res Commun; Mitochondrial function is impaired in yeast and human cellular models of Shwachman Diamond syndrome.; Authors - Henson Adrianna L AL
human	WB	Pediatr Blood Cancer; Impaired growth, hematopoietic colony formation, and ribosome maturation in human cells depleted of Shwachman-Diamond syndrome protein SBDS.; Authors - Sezgin Gulay G
human	WB	Evid Based Complement Alternat Med; Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins.; Authors - Meina Shi