Anticorps Monoclonal anti-EIF6

EIF6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2A8

N° de cat : 68765-3-PBS

Synonymes

integrin beta 4 binding protein, CAB, B4 integrin interactor, B(2)GCN homolog, b(2)gcn

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Galerie de données de validation

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  WB analysis using 68765-3-Ig (same clone as 68765-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68765-3-Ig (EIF6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68765-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68765-3-Ig (same clone as 68765-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF6 antibody (68765-3-Ig, Clone: 1F2A8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68765-3-PBS in a different storage buffer formulation.

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Informations sur le produit

68765-3-PBS cible EIF6 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF6 Protéine recombinante Ag30249
• Nom complet: eukaryotic translation initiation factor 6
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001119
• Symbole du gène: EIF6
• Identification du gène (NCBI): 3692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), it functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.

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