Anticorps Recombinant de lapin anti-EIF6

• Phare
• Validé par KD/KO

EIF6 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G23

N° de cat : 82693-1-PBS

Synonymes

integrin beta 4 binding protein, 2G23, b, b(2)gcn, B(2)GCN homolog

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Galerie de données de validation

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  WB analysis using 82693-1-RR (same clone as 82693-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82693-1-RR (EIF6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82693-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82693-1-RR (same clone as 82693-1-PBS)

  WB result of EIF6 antibody (82693-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF6 transfected HeLa cells. This data was developed using the same antibody clone with 82693-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82693-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82693-1-RR against Human EIF6 were performed. The affinity constant is 6.29 nM.

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Informations sur le produit

82693-1-PBS cible EIF6 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EIF6 Protéine recombinante Ag0324
• Nom complet: eukaryotic translation initiation factor 6
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001119
• Symbole du gène: EIF6
• Identification du gène (NCBI): 3692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), it functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.

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