Anticorps Recombinant de lapin anti-ELAC2

• Phare
• Validé par KD/KO

ELAC2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240487B6

N° de cat : 83468-6-RR

Synonymes

240487B6, EC:3.1.26.11, elaC homolog 2 (E. coli), ElaC homolog protein 2, ELC2

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Galerie de données de validation

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  WB analysis using 83468-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 83468-6-RR

  WB result of ELAC2 antibody (83468-6-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELAC2 transfected MCF-7 cells.

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  FC experiment of A431 using 83468-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83468-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules DU 145, cellules HeLa, cellules MCF-7, cellules Raji
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83468-6-RR cible ELAC2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ELAC2 Protéine recombinante Ag0105
• Nom complet: elaC homolog 2 (E. coli)
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC004158
• Symbole du gène: ELAC2
• Identification du gène (NCBI): 60528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

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