Anticorps Recombinant de lapin anti-ELAVL2

ELAVL2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251573E1

N° de cat : 86623-3-RR

Synonymes

ELAV2, ELAVL 2, ELAV-like neuronal protein 1, ELAV-like protein 2, Hu-antigen B

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Galerie de données de validation

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  WB analysis using 86623-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86623-3-RR (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86623-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86623-3-RR against Human ELAVL2 were performed. The affinity constant is 0.424 nM.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Neuro-2a, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris, tissu de moelle épinière de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86623-3-RR cible ELAVL2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ELAVL2 Protéine recombinante Ag5105
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC030692
• Symbole du gène: ELAVL2
• Identification du gène (NCBI): 1993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.

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