Anticorps Polyclonal de lapin anti-HuC

• Phare
• Validé par KD/KO

HuC Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (4)

N° de cat : 55047-1-AP

Synonymes

ELAVL 3, ELAVL3, ELAV-like protein 3, Hu antigen C, Hu-antigen C

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Galerie de données de validation

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  WB analysis using 55047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55047-1-AP (HuC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Neuro-2a using 55047-1-AP

  IP result of anti-HuC (IP:55047-1-AP, 4ug; Detection:55047-1-AP 1:5000) with Neuro-2a cells lysate 1440 ug.

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  IHC staining of human brain using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 55047-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Neuro-2a
• Résultats positifs en IP: cellules Neuro-2a,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• RIP: See 1 publications below

Informations sur le produit

55047-1-AP cible HuC dans les applications de WB, IHC, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: NM_001420
• Symbole du gène: HuC
• Identification du gène (NCBI): 1995
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.

Publications

Species	Application	Title
human	WB,IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
human	WB,IF,RIP	Nucleic Acids Res; HuB and HuD repress telomerase activity by dissociating HuR from TERC.; Authors - Xiaolei Cheng
zebrafish	IF	Invest Ophthalmol Vis Sci; Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function; Authors - Hou-Zhi Yang
human	WB	Am J Pathol; Anaplastic lymphoma kinase (ALK) overexpression is associated with aggressive phenotypic characteristics of ovarian high-grade serous carcinoma.; Authors - Toshihide Matsumoto
human	IF	Cell Stem Cell; KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury; Authors - Jesse D Lai
human	WB	iScience; Multivalent GU-rich oligonucleotides sequester TDP-43 in the nucleus by inducing high molecular weight RNP complexes; Authors - Xi Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Raquel (Verified Customer) (06-21-2024)

Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs using 55047-1-AP . HuC antibody at dilution of 1:50 (under 20x lens). (Green: HuC; Blue: DAPI)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Brain organoid made with human iPSCs

HuC Antibody Immunofluorescence validation (1:50 dilution) in Brain organoid made with human iPSCs (Cat no:55047-1-AP)

FH

Etienne (Verified Customer) (12-19-2022)

Did not work. Virtually only aspecific binding in human brain organoids

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/20, 1/50, 1/200
• Cell Tissue Type: Human Brain Organoids

HuC Antibody Immunofluorescence validation (1/20, 1/50, 1/200 dilution) in Human Brain Organoids (Cat no:55047-1-AP)

FH

Alessandro (Verified Customer) (11-07-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence

FH

David (Verified Customer) (09-01-2022)

Works good!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human brain

HuC Antibody Immunofluorescence validation (1:150 dilution) in Human brain (Cat no:55047-1-AP)

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