Anticorps Polyclonal de lapin anti-HuD-specific

HuD-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 24992-1-AP

Synonymes

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, HuD-specific, PNEM

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Galerie de données de validation

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  WB analysis of mouse brain using 24992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 24992-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 24992-1-AP

  IP Result of anti-HuD-specific (IP:24992-1-AP, 4ug; Detection:24992-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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  IHC staining of human brain using 24992-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 24992-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 24992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 24992-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 24992-1-AP (HuD-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

24992-1-AP cible HuD-specific dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HuD-specific Protéine recombinante Ag21830
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Masse moléculaire calculée: 380 aa, 42 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC036071
• Symbole du gène: ELAVL4
• Identification du gène (NCBI): 1996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 is expressed in brain and localizes in the nucleus. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

Publications

Species	Application	Title
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
human	WB	Prog Neurobiol; RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons.; Authors - Robert J van der Linden
human	WB,RIP	FASEB J; ELAVL4 promotes the tumorigenesis of small cell lung cancer by stabilizing LncRNA LYPLAL1-DT and enhancing profilin 2 activation; Authors - Shuxin Li