Anticorps Monoclonal anti-ELAVL4

ELAVL4 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B1F5

N° de cat : CL488-67835

Synonymes

HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4

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Galerie de données de validation

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  IF Staining of mouse brain using CL488-67835

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ELAVL4 antibody (CL488-67835, Clone: 1B1F5 ) at dilution of 1:200.

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  IF Staining of U2OS using CL488-67835

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 ELAVL4 antibody (CL488-67835, Clone: 1B1F5 ) at dilution of 1:200.

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  FC experiment of U2OS using CL488-67835

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ELAVL4 (CL488-67835, Clone:1B1F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67835 cible ELAVL4 dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ELAVL4 Protéine recombinante Ag3678
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Masse moléculaire calculée: 380 aa, 42 kDa
• Poids moléculaire observé: 32-40 kDa
• Numéro d’acquisition GenBank: BC036071
• Symbole du gène: ELAVL4
• Identification du gène (NCBI): 1996
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

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