Anticorps Polyclonal de lapin anti-ELF1

ELF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22565-1-PBS

Synonymes

ETS-related transcription factor Elf-1, E74-like factor 1, E74 like factor 1

---

Galerie de données de validation

• 

  WB analysis using 22565-1-AP (same clone as 22565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 22565-1-AP (same clone as 22565-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 22565-1-AP (same clone as 22565-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 22565-1-AP (same clone as 22565-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 22565-1-AP (same clone as 22565-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 22565-1-AP (same clone as 22565-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 22565-1-AP (same clone as 22565-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 22565-1-AP (same clone as 22565-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22565-1-AP (same clone as 22565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 22565-1-AP (same clone as 22565-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22565-1-AP (ELF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22565-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

22565-1-PBS cible ELF1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELF1 Protéine recombinante Ag14689
• Nom complet: E74-like factor 1 (ets domain transcription factor)
• Masse moléculaire calculée: 619 aa, 67 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC030507
• Symbole du gène: ELF1
• Identification du gène (NCBI): 1997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.

{{ptg:RelatedPrimaryAntibodies}}